# hmmsearch :: search profile(s) against a sequence database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: pfam_list/UPF0020.hmm.txt # target sequence database: proteomes/Saccharomyces_cerevisiae.fasta # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: UPF0020 [M=197] Accession: PF01170.17 Description: Putative RNA methylase family UPF0020 Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 8.2e-12 43.7 0.0 1.4e-10 39.7 0.0 2.2 1 sp|Q12463|TRM11_YEAST tRNA (guanine(10)-N2)-methyltransferas 1.9e-06 26.2 0.1 2.4e-05 22.6 0.1 2.2 1 sp|Q12052|TGS1_YEAST Trimethylguanosine synthase OS=Sacchar 0.0019 16.4 0.0 0.055 11.6 0.0 2.1 2 sp|Q03920|MTQ2_YEAST eRF1 methyltransferase catalytic subun ------ inclusion threshold ------ 0.015 13.5 0.0 0.039 12.1 0.0 1.6 1 sp|P40516|EFM4_YEAST Protein-lysine N-methyltransferase EFM 0.033 12.3 0.1 0.12 10.5 0.1 2.0 2 sp|P25087|ERG6_YEAST Sterol 24-C-methyltransferase OS=Sacch Domain annotation for each sequence (and alignments): >> sp|Q12463|TRM11_YEAST tRNA (guanine(10)-N2)-methyltransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 39.7 0.0 1.1e-13 1.4e-10 3 134 .. 184 297 .. 183 303 .. 0.79 Alignments for each domain: == domain 1 score: 39.7 bits; conditional E-value: 1.1e-13 SS-SS---SS-HHHHHHHHHHTT--TTS-EEETT-TTSHHHHHHHHHHTT--TTTT----SSHHHHHHHHHHHH--EEEEES-HHHHH CS UPF0020 3 yRefngpapLketLAaalvrlagwkpgepllDPmCGsGtilIEaallganiapgllrefvlelkaeaeeearaelklygsDldrrvvq 90 +R+++g ++ ++L+ + ++a++kpg+ ++DP+ G+G++l+ ++ g+ ++ gsD+d r+++ sp|Q12463|TRM11_YEAST 184 KRPYKGTTSFEAELSLVSANIAQVKPGTIMYDPFAGTGSFLVAGGHFGSLVI--------------------------GSDIDGRMIR 245 79***********************************************999..........................*********7 PP ..HHHHHHHHT..T-G...GGEEEEE--GGG--.STTS---EEEE------- CS UPF0020 91 ..gareNaeka..gvg...dliefsqadaakLr.lkegevdvivtnpPYGer 134 ga+ N ++ + g ++ ++ ++d+++ +++ +d+i+++pPYG+r sp|Q12463|TRM11_YEAST 246 gkGAQVNISANfkKYGessQFLDVLTMDFTNNAlRNNLVIDTILCDPPYGIR 297 3369988654311333001444555555554333366699**********99 PP >> sp|Q12052|TGS1_YEAST Trimethylguanosine synthase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 22.6 0.1 2e-08 2.4e-05 30 132 .. 99 179 .. 81 231 .. 0.83 Alignments for each domain: == domain 1 score: 22.6 bits; conditional E-value: 2e-08 S-EEETT-TTSHHHHHHHHHHTT--TTTT----SSHHHHHHHHHHHH--EEEEES-HHHHHHHHHHHHHTT-GGGEEEEE--GGG--.. CS UPF0020 30 epllDPmCGsGtilIEaallganiapgllrefvlelkaeaeeearaelklygsDldrrvvqgareNaekagvgdliefsqadaakLr.. 116 e++lD +CG+G+ I a++ +++ g+D +++ ++ +Na+++gv+d+i ++ +kL sp|Q12052|TGS1_YEAST 99 ERILDVFCGGGGNTIQFAMQFPYVY--------------------------GVDYSIEHIYCTAKNAQSYGVDDRIWLKRGSWKKLVsk 161 5566666666666666666666665..........................***********************************977 PP ..STTS---EEEE----- CS UPF0020 117 ..lkegevdvivtnpPYG 132 l++ ++d + + pP+G sp|Q12052|TGS1_YEAST 162 qkLSKIKYDCVFGSPPWG 179 7555669**********9 PP >> sp|Q03920|MTQ2_YEAST eRF1 methyltransferase catalytic subunit MTQ2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 11.6 0.0 4.6e-05 0.055 79 131 .. 73 125 .. 29 128 .. 0.79 2 ? 2.7 0.0 0.024 28 142 179 .. 156 193 .. 150 208 .. 0.83 Alignments for each domain: == domain 1 score: 11.6 bits; conditional E-value: 4.6e-05 EEEEES-HHHHHHHHHHHH.HTT-GGGEEEEE--GGG--STTS---EEEE---- CS UPF0020 79 lygsDldrrvvqgareNae.kagvgdliefsqadaakLrlkegevdvivtnpPY 131 ++D+++ +++++ + a+ + + + + e+ qad + ++++vdv++ npPY sp|Q03920|MTQ2_YEAST 73 HLAVDINPWALEATLDTAKlNSCKSSFLEVIQADLNSSI-RNNQVDVLIFNPPY 125 578999999999998888745677788899998887655.6779********** PP == domain 2 score: 2.7 bits; conditional E-value: 0.024 HHHHHHHHHHHHHH-TT..-EEEEEESSHHHHHCC--- CS UPF0020 142 ekLYseflrelkrvlrgggrlvlltsenkalekaleer 179 ++++++lr+l+++l+ +g + +l+ ++++ ++++++ sp|Q03920|MTQ2_YEAST 156 MAITDKLLRQLEQILSPDGVAYILFCARNKPKEVIKRF 193 5678999*********************9999999876 PP >> sp|P40516|EFM4_YEAST Protein-lysine N-methyltransferase EFM4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 12.1 0.0 3.3e-05 0.039 75 115 .. 116 156 .. 107 168 .. 0.81 Alignments for each domain: == domain 1 score: 12.1 bits; conditional E-value: 3.3e-05 HH--EEEEES-HHHHHHHHHHHHHTT-GGGEEEEE--GGG- CS UPF0020 75 aelklygsDldrrvvqgareNaekagvgdliefsqadaakL 115 + kl+g+D ++ v+ a + ae++gv+++i+f+qad+ + sp|P40516|EFM4_YEAST 116 FQGKLVGIDYSEESVKLASNIAEATGVDNFISFQQADIFSG 156 44569********************************8765 PP >> sp|P25087|ERG6_YEAST Sterol 24-C-methyltransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=55929 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 10.5 0.1 9.6e-05 0.12 76 125 .. 142 191 .. 101 217 .. 0.87 2 ? -3.5 0.0 2 2.4e+03 88 106 .. 256 274 .. 234 280 .. 0.77 Alignments for each domain: == domain 1 score: 10.5 bits; conditional E-value: 9.6e-05 H--EEEEES-HHHHHHHHHHHHHTT-GGGEEEEE--GGG--STTS---EE CS UPF0020 76 elklygsDldrrvvqgareNaekagvgdliefsqadaakLrlkegevdvi 125 +++g++++ +++a+ a+k++++d+++f++ d+ k+ +e+ +d + sp|P25087|ERG6_YEAST 142 GCNVIGLNNNDYQIAKAKYYAKKYNLSDQMDFVKGDFMKMDFEENTFDKV 191 45699***********************************9666678765 PP == domain 2 score: -3.5 bits; conditional E-value: 2 HHHHHHHHHHHTT-GGGEE CS UPF0020 88 vvqgareNaekagvgdlie 106 +v+ ar+ ++++g + l++ sp|P25087|ERG6_YEAST 256 HVDVARKALKNCGFEVLVS 274 5888999999999887775 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (197 nodes) Target sequences: 6049 (2936363 residues searched) Passed MSV filter: 168 (0.0277732); expected 121.0 (0.02) Passed bias filter: 137 (0.0226484); expected 121.0 (0.02) Passed Vit filter: 18 (0.0029757); expected 6.0 (0.001) Passed Fwd filter: 5 (0.000826583); expected 0.1 (1e-05) Initial search space (Z): 6049 [actual number of targets] Domain search space (domZ): 5 [number of targets reported over threshold] # CPU time: 0.06u 0.00s 00:00:00.06 Elapsed: 00:00:00.03 # Mc/sec: 19282.12 // [ok]