# hmmsearch :: search profile(s) against a sequence database # HMMER 3.1b2 (February 2015); http://hmmer.org/ # Copyright (C) 2015 Howard Hughes Medical Institute. # Freely distributed under the GNU General Public License (GPLv3). # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: pfam_list/UPF0020.hmm.txt # target sequence database: proteomes/Strongylocentrotus_purpuratus.fasta # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: UPF0020 [M=197] Accession: PF01170.17 Description: Putative RNA methylase family UPF0020 Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 9e-36 124.1 0.0 1.6e-34 120.0 0.0 2.1 1 tr|W4XM22|W4XM22_STRPU Uncharacterized protein OS=Strongyloc 7.3e-13 49.3 0.0 1.6e-12 48.2 0.0 1.5 1 tr|W4XEL6|W4XEL6_STRPU Uncharacterized protein OS=Strongyloc 3.1e-06 27.7 0.1 1.9e-05 25.1 0.1 2.1 1 tr|W4Y0S2|W4Y0S2_STRPU Uncharacterized protein OS=Strongyloc 5.1e-06 27.0 0.4 4.2e-05 24.0 0.4 2.1 1 tr|W4YQG0|W4YQG0_STRPU Uncharacterized protein OS=Strongyloc 0.00012 22.5 0.0 0.00016 22.1 0.0 1.0 1 tr|W4YXY3|W4YXY3_STRPU Uncharacterized protein OS=Strongyloc 0.00061 20.2 0.0 0.002 18.5 0.0 1.9 2 tr|W4YT52|W4YT52_STRPU Uncharacterized protein OS=Strongyloc ------ inclusion threshold ------ 0.023 15.1 0.0 0.035 14.5 0.0 1.4 1 tr|W4Z8R2|W4Z8R2_STRPU Uncharacterized protein OS=Strongyloc 0.057 13.8 0.8 0.19 12.0 0.8 1.9 1 tr|W4YBQ4|W4YBQ4_STRPU Uncharacterized protein OS=Strongyloc 0.31 11.4 0.1 3.3 8.0 0.0 2.3 3 tr|W4XDE9|W4XDE9_STRPU Uncharacterized protein OS=Strongyloc Domain annotation for each sequence (and alignments): >> tr|W4XM22|W4XM22_STRPU Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 120.0 0.0 5.2e-38 1.6e-34 2 194 .. 32 212 .. 30 214 .. 0.81 Alignments for each domain: == domain 1 score: 120.0 bits; conditional E-value: 5.2e-38 SSS-SS---SS-HHHHHHHHHHTT--TTS-EEETT-TTSHHHHHHHHHHTT--TTTT----SSHHHHHHHHHHHH--EEEEES-HHH CS UPF0020 2 gyRefngpapLketLAaalvrlagwkpgepllDPmCGsGtilIEaallganiapgllrefvlelkaeaeeearaelklygsDldrrv 88 ++++f g ++Lk+t+A++++rla+ kpge ++DPmCG G+i++E+ + +++++ ++ D++ + tr|W4XM22|W4XM22_STRPU 32 NITHF-GITTLKSTIAYCMLRLAQPKPGEIIMDPMCGVGGIPLEGLIDWKSCY------------------------VVSGDMNDVA 93 45555.599********************************************........................4455555555 PP HHHHHHHHHHT..........T-GGGEEEEE--GGG--STTS---EEEE----------HHHHHHHHHHHHHHHHHH-TT...-EEE CS UPF0020 89 vqgareNaeka..........gvgdliefsqadaakLrlkegevdvivtnpPYGerlgskkalekLYseflrelkrvlrgg.grlvl 164 v++++ N+ + + + + ++ ++d+++L+lk+++vdv vt++P+G+rlg+ k++++LY+++l+e++rv+r g gr+vl tr|W4XM22|W4XM22_STRPU 94 VERTKANLDSLaerrrekhieRPDIENDVLKWDVTNLPLKSSSVDVFVTDLPFGKRLGNRKNNWSLYHKCLEEMARVCRPGiGRAVL 180 5555555433211100000002223346789*******9999********************************************* PP EEESSHHHHHCC---..ESEEEEEEETTEEEE CS UPF0020 165 ltsenkalekaleer..alrkvkeknvklgll 194 lt+++k++ + l++ +rk+++ ++++g+l tr|W4XM22|W4XM22_STRPU 181 LTQDKKCMVQTLQRChhIWRKKTVTFINHGGL 212 ************998779*********99987 PP >> tr|W4XEL6|W4XEL6_STRPU Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 48.2 0.0 5e-16 1.6e-12 3 139 .. 243 378 .. 242 383 .. 0.82 Alignments for each domain: == domain 1 score: 48.2 bits; conditional E-value: 5e-16 SS-SS---SS-HHHHHHHHHHTT--TTS-EEETT-TTSHHHHHHHHHHTT--TTTT----SS...HHHHHHHHHHHH--EEEEES-H CS UPF0020 3 yRefngpapLketLAaalvrlagwkpgepllDPmCGsGtilIEaallganiapgllrefvle...lkaeaeeearaelklygsDldr 86 R+f g++++ + L+ ++ + ag+++g+ lDP+ G+G++l+ aa g ++ + ++++ + ++a+ +++++ k +g D + tr|W4XEL6|W4XEL6_STRPU 243 TRHFIGNTSMDALLSLVMTNQAGVREGDLVLDPFVGTGSLLVAAAHFGGYVSGCDINYNIIHgrgKSSRAQPKTETKTKYRGRDENI 329 69*************************************************9655655555588888999999999999*****886 PP HHHHHHHHHHHHTT-GGGE.EEEE--G.GG--STTS---EEEE----------HH CS UPF0020 87 rvvqgareNaekagvgdli.efsqada.akLrlkegevdvivtnpPYGerlgskk 139 + N+e++g+g++ ++ d+ +++ +++ +d+i+t+pPYG+r ++ k tr|W4XEL6|W4XEL6_STRPU 330 S------ANLEQYGLGHRYlDVLISDFsQDIWREQPLFDAIITDPPYGIREKTRK 378 5......69*****99974122222321445556668*************88765 PP >> tr|W4Y0S2|W4Y0S2_STRPU Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 25.1 0.1 5.9e-09 1.9e-05 30 132 .. 1294 1369 .. 1286 1395 .. 0.90 Alignments for each domain: == domain 1 score: 25.1 bits; conditional E-value: 5.9e-09 S-EEETT-TTSHHHHHHHHHHTT--TTTT----SSHHHHHHHHHHHH--EEEEES-HHHHHHHHHHHHHTT-GGGEEEEE--GGG CS UPF0020 30 epllDPmCGsGtilIEaallganiapgllrefvlelkaeaeeearaelklygsDldrrvvqgareNaekagvgdliefsqadaak 114 + ++D +CGsG+ +I a ++++ ++D+d++ ++ ar Na +gv+d+ief + d+ k tr|W4Y0S2|W4Y0S2_STRPU 1294 DLIVDAFCGSGGNAIQFAFTCERVV--------------------------AVDIDPAKIELARHNATVYGVEDRIEFIVGDFFK 1352 5688999999999999999988888..........................9********************************* PP --STTS---EEEE----- CS UPF0020 115 LrlkegevdvivtnpPYG 132 + + + dv+ pP+G tr|W4Y0S2|W4Y0S2_STRPU 1353 VA-DYLKADVVFLSPPWG 1369 **.88899*********9 PP >> tr|W4YQG0|W4YQG0_STRPU Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 24.0 0.4 1.3e-08 4.2e-05 50 136 .. 70 132 .. 24 149 .. 0.78 Alignments for each domain: == domain 1 score: 24.0 bits; conditional E-value: 1.3e-08 HTT--TTTT----SSHHHHHHHHHHHH--EEEEES-HHHHHHHHHHHHHTT-GGGEEEEE--GGG--..STTS---EEEE------- CS UPF0020 50 ganiapgllrefvlelkaeaeeearaelklygsDldrrvvqgareNaekagvgdliefsqadaakLr..lkegevdvivtnpPYGer 134 a +g+D+d+ +++ +++N e+ ++ ++ +q d +k++ ++++d+++ npP+G + tr|W4YQG0|W4YQG0_STRPU 70 DAGL-------------------------CIGFDIDQDALEICQRNCEEFELP-CVDTVQSDLTKMEagPWSKKFDTVIMNPPFGTK 130 4444.........................48********************98.5777777*****98745669***********87 PP -- CS UPF0020 135 lg 136 + tr|W4YQG0|W4YQG0_STRPU 131 HN 132 55 PP >> tr|W4YXY3|W4YXY3_STRPU Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 22.1 0.0 5e-08 0.00016 3 39 .. 16 52 .. 15 52 .. 0.97 Alignments for each domain: == domain 1 score: 22.1 bits; conditional E-value: 5e-08 SS-SS---SS-HHHHHHHHHHTT--TTS-EEETT-TT CS UPF0020 3 yRefngpapLketLAaalvrlagwkpgepllDPmCGs 39 R+f g++++ + L+ ++ + ag+++g+ lDP+ G+ tr|W4YXY3|W4YXY3_STRPU 16 TRHFIGNTSMDALLSLVMTNQAGVQEGDLVLDPFVGT 52 69*********************************98 PP >> tr|W4YT52|W4YT52_STRPU Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? -3.4 0.0 3.2 1e+04 79 116 .. 170 205 .. 153 212 .. 0.62 2 ! 18.5 0.0 6.4e-07 0.002 76 132 .. 269 331 .. 222 338 .. 0.78 Alignments for each domain: == domain 1 score: -3.4 bits; conditional E-value: 3.2 EEEEES-HHHHHHHHHHHHHTT-GGGEEEEE--GGG-- CS UPF0020 79 lygsDldrrvvqgareNaekagvgdliefsqadaakLr 116 + + +rv++ a +++ a+v + + ++d+++L+ tr|W4YT52|W4YT52_STRPU 170 ILSTEERSRVMELASQKL--ARVPMQYILGEWDFRDLT 205 566666666666666655..445555556777777777 PP == domain 2 score: 18.5 bits; conditional E-value: 6.4e-07 H--EEEEES-HHHHHHHHHHHHHTT-GGGEEEEE--GGG...--...STTS.---EEEE----- CS UPF0020 76 elklygsDldrrvvqgareNaekagvgdliefsqadaak...Lr...lkeg.evdvivtnpPYG 132 +++ ++D r +v +++Na++ gv+++ ++ ++ Lr + g +dviv+npPY tr|W4YT52|W4YT52_STRPU 269 LAHVTAIDASRDAVMLTQDNASRLGVSNRLSVHHTALTDespLRihsKY-GtRYDVIVSNPPYL 331 45599***********************999887655432227775433.349**********6 PP >> tr|W4Z8R2|W4Z8R2_STRPU Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 14.5 0.0 1.1e-05 0.035 75 123 .. 65 113 .. 55 151 .. 0.90 Alignments for each domain: == domain 1 score: 14.5 bits; conditional E-value: 1.1e-05 HH--EEEEES-HHHHHHHHHHHHHTT-GGGEEEEE--GGG--STTS--- CS UPF0020 75 aelklygsDldrrvvqgareNaekagvgdliefsqadaakLrlkegevd 123 +e+++ D+++ +++ +++ a++ g++ i++++ +a +L+ +++++d tr|W4Z8R2|W4Z8R2_STRPU 65 VEQHVTVCDINEDMLRVGKKRAKQRGIQSGISWVCGNAEELPIADDSMD 113 56668889**********************************7777887 PP >> tr|W4YBQ4|W4YBQ4_STRPU Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=3 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 12.0 0.8 6e-05 0.19 22 92 .. 510 573 .. 494 597 .. 0.82 Alignments for each domain: == domain 1 score: 12.0 bits; conditional E-value: 6e-05 HHTT--TTS-EEETT-TTSHHHHHHHHHHTT--TTTT----SSHHHHHHHHHHHH--EEEEES-HHHHHHH CS UPF0020 22 rlagwkpgepllDPmCGsGtilIEaallganiapgllrefvlelkaeaeeearaelklygsDldrrvvqga 92 a++++ ++llD CG+Gti I a +++++ +e+ ++a e+a++++ + g+D+ + +a tr|W4YBQ4|W4YBQ4_STRPU 510 DWAQVTKITTLLDICCGTGTIGISLAKRVEKVI-------GVEMNEQAIEDAKENAAINGVDNVTFHCGKA 573 5678888899*****************999999.......4567888888888888899998876655555 PP >> tr|W4XDE9|W4XDE9_STRPU Uncharacterized protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ? 8.0 0.0 0.001 3.3 79 128 .. 31 77 .. 16 81 .. 0.82 2 ? -2.6 0.0 1.9 6.1e+03 20 41 .. 233 254 .. 231 254 .. 0.88 3 ? -0.0 0.0 0.3 9.5e+02 79 125 .. 267 312 .. 258 315 .. 0.70 Alignments for each domain: == domain 1 score: 8.0 bits; conditional E-value: 0.001 EEEEES-HHHHHHHHHHHHHTT-GGGEEEEE--GGG--STTS---EEEE- CS UPF0020 79 lygsDldrrvvqgareNaekagvgdliefsqadaakLrlkegevdvivtn 128 + ++D+ ++ v++ N e g + i+f+qad+ +L l+ +++d+i +n tr|W4XDE9|W4XDE9_STRPU 31 VTAVDFMPSFVEK---NREINGHMGNIDFKQADVMALDLPLNSYDIIFSN 77 5667777777764...6677788899************9999*****999 PP == domain 2 score: -2.6 bits; conditional E-value: 1.9 HHHHTT--TTS-EEETT-TTSH CS UPF0020 20 lvrlagwkpgepllDPmCGsGt 41 +v+l g++++++ lD CG G+ tr|W4XDE9|W4XDE9_STRPU 233 FVELLGLQEDQTVLDVGCGIGG 254 688999*********9999885 PP == domain 3 score: -0.0 bits; conditional E-value: 0.3 EEEEES-HHHHHHHHHHHHHTT-GGGEEEEE--GGG--STTS---EE CS UPF0020 79 lygsDldrrvvqgareNaekagvgdliefsqadaakLrlkegevdvi 125 + g+Dl +++++ a + a+ + + + f+ d++k + + +++dvi tr|W4XDE9|W4XDE9_STRPU 267 VDGIDLSSNMIEVAMDRAQGQNQPK-VIFEIGDITKREYSPESFDVI 312 679*************998765544.445666677666555578877 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (197 nodes) Target sequences: 28594 (14266044 residues searched) Passed MSV filter: 783 (0.0273834); expected 571.9 (0.02) Passed bias filter: 609 (0.0212982); expected 571.9 (0.02) Passed Vit filter: 54 (0.00188851); expected 28.6 (0.001) Passed Fwd filter: 9 (0.000314751); expected 0.3 (1e-05) Initial search space (Z): 28594 [actual number of targets] Domain search space (domZ): 9 [number of targets reported over threshold] # CPU time: 0.22u 0.01s 00:00:00.23 Elapsed: 00:00:00.09 # Mc/sec: 31226.79 // [ok]