# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.1b2 (February 2015); http://hmmer.org/
# Copyright (C) 2015 Howard Hughes Medical Institute.
# Freely distributed under the GNU General Public License (GPLv3).
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file: pfam_list/UPF0020.hmm.txt
# target sequence database: proteomes/Tetrahymena_thermophila.fasta
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Query: UPF0020 [M=197]
Accession: PF01170.17
Description: Putative RNA methylase family UPF0020
Scores for complete sequences (score includes all domains):
--- full sequence --- --- best 1 domain --- -#dom-
E-value score bias E-value score bias exp N Sequence Description
------- ------ ----- ------- ------ ----- ---- -- -------- -----------
2.4e-09 37.8 0.0 5.4e-08 33.3 0.0 2.6 2 tr|Q23CZ3|Q23CZ3_TETTS Methyltransferase domain protein OS=T
0.00031 21.0 0.0 0.00055 20.3 0.0 1.3 1 tr|W7XD08|W7XD08_TETTS tRNA (guanine(37)-N1)-methyltransfera
0.0078 16.5 0.1 0.01 16.1 0.1 1.2 1 tr|Q23EX7|Q23EX7_TETTS Methyltransferase small domain protei
------ inclusion threshold ------
0.03 14.6 0.1 0.13 12.6 0.1 2.1 1 tr|Q22MG6|Q22MG6_TETTS RNA cap guanine-N2 methyltransferase
0.035 14.4 0.0 0.051 13.8 0.0 1.3 1 tr|W7XCF3|W7XCF3_TETTS HemK methyltransferase family protein
0.053 13.8 0.2 0.13 12.5 0.2 1.6 1 tr|I7MDG3|I7MDG3_TETTS Serine/Threonine kinase domain protei
0.1 12.8 0.0 0.27 11.5 0.0 1.6 2 tr|Q24GH8|Q24GH8_TETTS rRNA adenine N(6)-methyltransferase O
1.7 8.9 7.9 0.9 9.8 1.4 3.2 2 tr|I7M9L2|I7M9L2_TETTS Uncharacterized protein OS=Tetrahymen
Domain annotation for each sequence (and alignments):
>> tr|Q23CZ3|Q23CZ3_TETTS Methyltransferase domain protein OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=TTHER
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 33.3 0.0 1.6e-11 5.4e-08 4 141 .. 248 385 .. 246 390 .. 0.81
2 ? 1.0 0.0 0.13 4.3e+02 157 188 .. 534 565 .. 524 573 .. 0.85
Alignments for each domain:
== domain 1 score: 33.3 bits; conditional E-value: 1.6e-11
S-SS---SS-HHHHHHHHHHTT--TTS-EEETT-TTSHHHHHHHHHHTT--TTTT----SS....HHHHHHHHHHHH--EEEEES-H CS
UPF0020 4 RefngpapLketLAaalvrlagwkpgepllDPmCGsGtilIEaallganiapgllrefvle....lkaeaeeearaelklygsDldr 86
R + gp++ + L+ + + + g+++lDP+ GsG++ I ga + g l ++vl+ + + + ++ ++ + y+ ++++
tr|Q23CZ3|Q23CZ3_TETTS 248 RAYLGPTSTDNILSFLMCNQGLTRSGDMILDPFVGSGSLIIPPSHFGAICFGGDLDPRVLHgfgvGRLNKKSQFYQKNEEYCKEMKP 334
78888899999999999999999****************************99998888888876566666666678889*****99 PP
HHHHHHHHHHHHTT-GG..GEEEEE--GGG--STTS---EEEE----------HHHH CS
UPF0020 87 rvvqgareNaekagvgd..liefsqadaakLrlkegevdvivtnpPYGerlgskkal 141
+++ N e++++++ +i + + +++Lr + + +d+i+++pPYG r + a
tr|Q23CZ3|Q23CZ3_TETTS 335 KIM----LNFEQYELNKpnIIRMDCI-NTNLR-EVEIFDAIICDPPYGFRAMTRTAG 385
996....6888887765115666664.56699.5558************99887665 PP
== domain 2 score: 1.0 bits; conditional E-value: 0.13
TT..-EEEEEESSHHHHHCC---ESEEEEEEE CS
UPF0020 157 rgggrlvlltsenkalekaleeralrkvkekn 188
++++r+++ +++nk+++++lee+ ++ e+n
tr|Q23CZ3|Q23CZ3_TETTS 534 EKKSRILVTLQKNKSVKEFLEEAGYKNLDEMN 565
45699******************999988887 PP
>> tr|W7XD08|W7XD08_TETTS tRNA (guanine(37)-N1)-methyltransferase OS=Tetrahymena thermophila (strain SB210) OX=312017 G
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 20.3 0.0 1.6e-07 0.00055 27 90 .. 222 285 .. 218 312 .. 0.90
Alignments for each domain:
== domain 1 score: 20.3 bits; conditional E-value: 1.6e-07
-TTS-EEETT-TTSHHHHHHHHHHTT--TTTT----SSHHHHHHHHHHHH--EEEEES-HHHHH CS
UPF0020 27 kpgepllDPmCGsGtilIEaallganiapgllrefvlelkaeaeeearaelklygsDldrrvvq 90
kp+e+ lD +CG G ++I aa +g +++ l ++ + + +e++++e k ++++d+r v
tr|W7XD08|W7XD08_TETTS 222 KPNETVLDLFCGVGPLAIRAAKMGCKVICNDLNPHCYDYLLINREKNNVEDKTLCFNNDARKVV 285
7999**********************************9999999**************98765 PP
>> tr|Q23EX7|Q23EX7_TETTS Methyltransferase small domain protein OS=Tetrahymena thermophila (strain SB210) OX=312017 GN
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 16.1 0.1 3.1e-06 0.01 76 147 .. 86 160 .. 44 177 .. 0.78
Alignments for each domain:
== domain 1 score: 16.1 bits; conditional E-value: 3.1e-06
H--EEEEES-HHHHHHHHHHHHHTT-GGGEEEEE--GGG--.STTS---EEEE----------HHHH..HHHHHH CS
UPF0020 76 elklygsDldrrvvqgareNaekagvgdliefsqadaakLr.lkegevdvivtnpPYGerlgskkal..ekLYse 147
+++D++ + +++ a+++ +++ +++ a L + +g+ dv+++npPY +++ a +kLY++
tr|Q23EX7|Q23EX7_TETTS 86 SSLAIAIDINYDANILTQKYANHYHLSQVECVNTDVAEGLYnRLKGQADVVICNPPYVPTEDEEVASayQKLYQK 160
3337788888888888889999999988777777667778888999***********988777665545888875 PP
>> tr|Q22MG6|Q22MG6_TETTS RNA cap guanine-N2 methyltransferase OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=T
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ? 12.6 0.1 3.7e-05 0.13 73 131 .. 397 453 .. 373 472 .. 0.85
Alignments for each domain:
== domain 1 score: 12.6 bits; conditional E-value: 3.7e-05
HHHH--EEEEES-HHHHHHHHHHHHHTT-GGGEEEEE--GGG--STTS---EEEE---- CS
UPF0020 73 araelklygsDldrrvvqgareNaekagvgdliefsqadaakLrlkegevdvivtnpPY 131
a++ +++++D ++ v a+ N e+ + ++++e+ q d+ +++ + + d++v p Y
tr|Q22MG6|Q22MG6_TETTS 397 AKEASQVIAIDCNQVKVSHAKYNIESFKQQHKVEVIQSDFLQMK--KLKADTVVLHPDY 453
3455669************************************9..3457999987766 PP
>> tr|W7XCF3|W7XCF3_TETTS HemK methyltransferase family protein OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ? 13.8 0.0 1.5e-05 0.051 75 131 .. 138 201 .. 110 222 .. 0.78
Alignments for each domain:
== domain 1 score: 13.8 bits; conditional E-value: 1.5e-05
HH--EEEEES-HHHHHHHHHHHHHTT-G....GGEEEEE--G..GG--.STTS---EEEE---- CS
UPF0020 75 aelklygsDldrrvvqgareNaekagvg....dliefsqada..akLr.lkegevdvivtnpPY 131
+ +++D+++ ++ a++N++ +++ +li+++++++ +L+ ++++++d iv+npPY
tr|W7XCF3|W7XCF3_TETTS 138 SQFTGIAIDKQKFCIELAKQNLNLNKIDpqrlQLIHLECLKFfqNNLNqNPDQQFDFIVSNPPY 201
44556789999999999999999887753333678888888722356677888*********** PP
>> tr|I7MDG3|I7MDG3_TETTS Serine/Threonine kinase domain protein OS=Tetrahymena thermophila (strain SB210) OX=312017 GN
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ? 12.5 0.2 3.9e-05 0.13 89 166 .. 97 173 .. 49 200 .. 0.80
Alignments for each domain:
== domain 1 score: 12.5 bits; conditional E-value: 3.9e-05
HHHHHHHHHHTT-GGGEEEEE--GGG--STTS---EEEE----------HHHHHHHHHHHHHHHHHH-TT..-EEEEE CS
UPF0020 89 vqgareNaekagvgdliefsqadaakLrlkegevdvivtnpPYGerlgskkalekLYseflrelkrvlrgggrlvllt 166
+ + N e+a+ ++l e+ ++a+ L+ k + d+ +n+ YG+rl++ + + +L+ e ++ +++ +++ r ++l
tr|I7MDG3|I7MDG3_TETTS 97 QIQNQANSENANKEELPEYLPLNACYLK-KIKYSDTSYANYKYGIRLQKGELKSELFTESAENFNKLYEEIKRFCILP 173
445689**********************.66689********************************999988888764 PP
>> tr|Q24GH8|Q24GH8_TETTS rRNA adenine N(6)-methyltransferase OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=TT
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ? 11.5 0.0 7.9e-05 0.27 75 133 .. 60 116 .. 45 121 .. 0.89
2 ? -3.3 0.1 2.7 9e+03 80 106 .. 222 248 .. 214 263 .. 0.58
Alignments for each domain:
== domain 1 score: 11.5 bits; conditional E-value: 7.9e-05
HH--EEEEES-HHHHHHHHHHHHHTT-GGGEEEEE--GGG--STTS---EEEE------ CS
UPF0020 75 aelklygsDldrrvvqgareNaekagvgdliefsqadaakLrlkegevdvivtnpPYGe 133
+ k++++++d+r+v+ ++ ++ + +++ e+ q da + + + +dv v+n PY +
tr|Q24GH8|Q24GH8_TETTS 60 KAKKVIAVEIDPRMVAELNKRFKYSQHAHKFELIQGDAISTEFPF--FDVCVANTPYQI 116
44569*********************************9888555..9*********76 PP
== domain 2 score: -3.3 bits; conditional E-value: 2.7
EEEES-HHHHHHHHHHHHHTT-GGGEE CS
UPF0020 80 ygsDldrrvvqgareNaekagvgdlie 106
++ ++++v+q ++N e ++ + ie
tr|Q24GH8|Q24GH8_TETTS 222 SAIFKNKSVLQVLEKNFETYQKIKAIE 248
444455555555555555555555554 PP
>> tr|I7M9L2|I7M9L2_TETTS Uncharacterized protein OS=Tetrahymena thermophila (strain SB210) OX=312017 GN=TTHERM_0050242
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ? 9.8 1.4 0.00027 0.9 62 119 .. 498 554 .. 476 565 .. 0.82
2 ? 0.5 0.6 0.18 6.1e+02 57 102 .. 910 956 .. 896 984 .. 0.84
Alignments for each domain:
== domain 1 score: 9.8 bits; conditional E-value: 0.00027
-SSHHHHHHHHHHHH--EEEEES-HHHHHHHHHHHHHTT-GGGEEEEE--GGG--STT CS
UPF0020 62 vlelkaeaeeearaelklygsDldrrvvqgareNaekagvgdliefsqadaakLrlke 119
+ + +++ +e+++++++ +++++ +q++++N + ++v++ ++++q ++ + + +e
tr|I7M9L2|I7M9L2_TETTS 498 NSSKNQKLDQEEQKQQQNNIKEIKQKTLQTSKSNTQLCQVKQNVKIQQQNNSQNK-PE 554
444667777788888889999***************************9987655.44 PP
== domain 2 score: 0.5 bits; conditional E-value: 0.18
TT----SSHHHHHHHHHH.HH--EEEEES-HHHHHHHHHHHHHTT-G CS
UPF0020 57 llrefvlelkaeaeeear.aelklygsDldrrvvqgareNaekagvg 102
+++++ ++l++++++e++ ++++ ++ ++ ++ vq +N ++ +v
tr|I7M9L2|I7M9L2_TETTS 910 FNQSQQTNLQNKIQQEINkVNQQGICQNITNEQVQNQIQNQKANQVP 956
5566667799999999997788899**********999999887765 PP
Internal pipeline statistics summary:
-------------------------------------
Query model(s): 1 (197 nodes)
Target sequences: 26971 (16912921 residues searched)
Passed MSV filter: 1136 (0.0421193); expected 539.4 (0.02)
Passed bias filter: 617 (0.0228764); expected 539.4 (0.02)
Passed Vit filter: 50 (0.00185384); expected 27.0 (0.001)
Passed Fwd filter: 8 (0.000296615); expected 0.3 (1e-05)
Initial search space (Z): 26971 [actual number of targets]
Domain search space (domZ): 8 [number of targets reported over threshold]
# CPU time: 0.27u 0.01s 00:00:00.28 Elapsed: 00:00:00.11
# Mc/sec: 30289.50
//
[ok]