# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.1b2 (February 2015); http://hmmer.org/
# Copyright (C) 2015 Howard Hughes Medical Institute.
# Freely distributed under the GNU General Public License (GPLv3).
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file: pfam_list/UPF0020.hmm.txt
# target sequence database: proteomes/Trypanosoma_brucei.fasta
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Query: UPF0020 [M=197]
Accession: PF01170.17
Description: Putative RNA methylase family UPF0020
Scores for complete sequences (score includes all domains):
--- full sequence --- --- best 1 domain --- -#dom-
E-value score bias E-value score bias exp N Sequence Description
------- ------ ----- ------- ------ ----- ---- -- -------- -----------
6.6e-08 31.4 0.1 7.9e-06 24.6 0.1 2.4 1 tr|Q57VW4|Q57VW4_TRYB2 Methyltransferase, putative OS=Trypan
0.0025 16.5 1.3 0.028 13.1 1.3 2.1 1 tr|Q4GYR8|Q4GYR8_TRYB2 Uncharacterized protein OS=Trypanosom
0.0098 14.5 0.1 0.041 12.5 0.0 1.9 3 tr|Q4FKJ2|Q4FKJ2_TRYB2 Methyltransferase OS=Trypanosoma bruc
------ inclusion threshold ------
0.039 12.6 0.0 0.061 11.9 0.0 1.3 1 tr|Q581Z0|Q581Z0_TRYB2 Histone-lysine N-methyltransferase, H
0.058 12.0 1.4 0.63 8.6 0.8 2.8 2 tr|Q389Z0|Q389Z0_TRYB2 Uncharacterized protein OS=Trypanosom
0.089 11.4 0.0 0.13 10.9 0.0 1.2 1 tr|Q4GZF2|Q4GZF2_TRYB2 Histone-lysine N-methyltransferase, H
Domain annotation for each sequence (and alignments):
>> tr|Q57VW4|Q57VW4_TRYB2 Methyltransferase, putative OS=Trypanosoma brucei brucei (strain 927/4 GUTat10.1) OX=185431 G
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 24.6 0.1 5.6e-09 7.9e-06 3 143 .. 356 576 .. 355 613 .. 0.89
Alignments for each domain:
== domain 1 score: 24.6 bits; conditional E-value: 5.6e-09
SS-SS---SS-HHHHHHHHHHTT--TTS-EEETT-TTSHHHHHHHHHHTT--TTTT----SS......................... CS
UPF0020 3 yRefngpapLketLAaalvrlagwkpgepllDPmCGsGtilIEaallganiapgllrefvle......................... 64
R++ g +++ ++ A ++ la +++g+ +DP+ G+ ++l+ aa ga +g + ++++
tr|Q57VW4|Q57VW4_TRYB2 356 CRPYIGTTSMPPEPAFVMAHLACVRRGSFVFDPFAGTCGVLVAAAHYGAVTFAGDVDGRAMQrgtqrglrsaqqrqqraaavralge 442
5899999999******************************************9*9999999999*********************** PP
......................................................HHHHHHHHHH...HH--EEEEES-HHHHHHHHH CS
UPF0020 65 ......................................................lkaeaeeear...aelklygsDldrrvvqgare 94
++++ ++++ +++ ++ + + v + +
tr|Q57VW4|Q57VW4_TRYB2 443 ealqragvpleealegptvrtnfkiyglnppervrmnfalwektlrlrgwgrggSLKNVAANFEgfgSTANGPCISNVTNDVSRDGS 529
******************************************************777777777778889999999999999999999 PP
HHHHTT-GGGEEEEE--GGG--STTS---EEEE----------HHHHHH CS
UPF0020 95 NaekagvgdliefsqadaakLrlkegevdvivtnpPYGerlgskkalek 143
N+ + +v+d + + a L eg d+ivt+pPYG+r +k a k
tr|Q57VW4|Q57VW4_TRYB2 530 NDASNNVSDGLGMAAVSEARLC--EGFLDAIVTDPPYGIREPRKSAAPK 576
9999999999999999999888..6679************888877654 PP
>> tr|Q4GYR8|Q4GYR8_TRYB2 Uncharacterized protein OS=Trypanosoma brucei brucei (strain 927/4 GUTat10.1) OX=185431 GN=TB
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 13.1 1.3 1.9e-05 0.028 21 101 .. 389 443 .. 379 460 .. 0.76
Alignments for each domain:
== domain 1 score: 13.1 bits; conditional E-value: 1.9e-05
HHHTT--TTS-EEETT-TTSHHHHHHHHHHTT--TTTT----SSHHHHHHHHHHHH--EEEEES-HHHHHHHHHHHHHTT- CS
UPF0020 21 vrlagwkpgepllDPmCGsGtilIEaallganiapgllrefvlelkaeaeeearaelklygsDldrrvvqgareNaekagv 101
v +a+++p+++llD CG+Gti I a +++++ g++l +++v+ ar Nae+ gv
tr|Q4GYR8|Q4GYR8_TRYB2 389 VAVAELTPETTLLDLCCGTGTIGIALAKHVKRVI--------------------------GIELVESAVRDARLNAERNGV 443
6789********************8888888887..........................555555555555555555554 PP
>> tr|Q4FKJ2|Q4FKJ2_TRYB2 Methyltransferase OS=Trypanosoma brucei brucei (strain 927/4 GUTat10.1) OX=185431 GN=Tb10.6k1
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ? -1.9 0.0 0.78 1.1e+03 95 128 .. 34 64 .. 21 92 .. 0.66
2 ? -2.4 0.0 1.1 1.5e+03 20 41 .. 96 117 .. 75 119 .. 0.80
3 ! 12.5 0.0 2.9e-05 0.041 76 123 .. 127 174 .. 117 178 .. 0.86
Alignments for each domain:
== domain 1 score: -1.9 bits; conditional E-value: 0.78
HHHHTT-GGGEEEEE--GGG--STTS---EEEE- CS
UPF0020 95 NaekagvgdliefsqadaakLrlkegevdvivtn 128
+++ g g + + + da++L+ + e+ ivt+
tr|Q4FKJ2|Q4FKJ2_TRYB2 34 RDRYDGKGASASERRQDATSLT--N-EYYDIVTD 64
5556666666777778899888..3.56667777 PP
== domain 2 score: -2.4 bits; conditional E-value: 1.1
HHHHTT--TTS-EEETT-TTSH CS
UPF0020 20 lvrlagwkpgepllDPmCGsGt 41
l + a+ kp+++ lD CG G+
tr|Q4FKJ2|Q4FKJ2_TRYB2 96 LAYHAQFKPTDTVLDVGCGIGG 117
6778999999999999999886 PP
== domain 3 score: 12.5 bits; conditional E-value: 2.9e-05
H--EEEEES-HHHHHHHHHHHHHTT-GGGEEEEE--GGG--STTS--- CS
UPF0020 76 elklygsDldrrvvqgareNaekagvgdliefsqadaakLrlkegevd 123
+++g+++++ +++ar+ ++++g++++i++ + d++++ ++e+d
tr|Q4FKJ2|Q4FKJ2_TRYB2 127 SCNVMGVNNNEYQINRARQHDSRYGMSGKINYTKTDFCNMCFGDNEFD 174
45699*********************************9986666666 PP
>> tr|Q581Z0|Q581Z0_TRYB2 Histone-lysine N-methyltransferase, H3 lysine-79 specific OS=Trypanosoma brucei brucei (strai
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ? 11.9 0.0 4.3e-05 0.061 12 71 .. 112 172 .. 104 198 .. 0.77
Alignments for each domain:
== domain 1 score: 11.9 bits; conditional E-value: 4.3e-05
S-HHHHHHHHHHTT--TTS-EEETT-TTSHHHHHHHHH.HTT--TTTT----SSHHHHHHH CS
UPF0020 12 LketLAaalvrlagwkpgepllDPmCGsGtilIEaall.ganiapgllrefvlelkaeaee 71
L +t + +vrl +++ +++++D CG G+il a l ga+++ + + e+ ++ ++a e
tr|Q581Z0|Q581Z0_TRYB2 112 LLPTFVSRMVRLMEITSEDTFYDLGCGNGSILFQVAFLtGARCVGIEISEHNAKVAKKAWE 172
66778889************************99885526777766666666665555544 PP
>> tr|Q389Z0|Q389Z0_TRYB2 Uncharacterized protein OS=Trypanosoma brucei brucei (strain 927/4 GUTat10.1) OX=185431 GN=Tb
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ? 8.6 0.8 0.00044 0.63 117 131 .. 300 314 .. 202 317 .. 0.58
2 ? -1.9 0.0 0.73 1e+03 40 62 .. 375 397 .. 368 415 .. 0.72
Alignments for each domain:
== domain 1 score: 8.6 bits; conditional E-value: 0.00044
STTS---EEEE---- CS
UPF0020 117 lkegevdvivtnpPY 131
+ g++d+iv+npPY
tr|Q389Z0|Q389Z0_TRYB2 300 EHAGSFDIIVSNPPY 314
5678*********** PP
== domain 2 score: -1.9 bits; conditional E-value: 0.73
SHHHHHHHHHHTT--TTTT---- CS
UPF0020 40 GtilIEaallganiapgllrefv 62
i IE +l+++++a + +++
tr|Q389Z0|Q389Z0_TRYB2 375 PNIVIEVGLQAEQVASVMEKSKL 397
56899999999999976665544 PP
>> tr|Q4GZF2|Q4GZF2_TRYB2 Histone-lysine N-methyltransferase, H3 lysine-79 specific OS=Trypanosoma brucei brucei (strai
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ? 10.9 0.0 8.8e-05 0.13 14 72 .. 98 156 .. 85 183 .. 0.67
Alignments for each domain:
== domain 1 score: 10.9 bits; conditional E-value: 8.8e-05
HHHHHHHHHHTT--TTS-EEETT-TTSHHHHHHHHH.HTT--TTTT----SSHHHHHHHH CS
UPF0020 14 etLAaalvrlagwkpgepllDPmCGsGtilIEaall.ganiapgllrefvlelkaeaeee 72
+ +A+ l+r+a+++++++++D CG G++l al ga+++ + e+ ++ +ea ++
tr|Q4GZF2|Q4GZF2_TRYB2 98 PFVAR-LLRVANVTADDTFYDLGCGNGSVLFHVALAtGASCVGVEINEHNAKVAKEAWTH 156
55554.89**********************999986256666556666666644444444 PP
Internal pipeline statistics summary:
-------------------------------------
Query model(s): 1 (197 nodes)
Target sequences: 8561 (4332363 residues searched)
Passed MSV filter: 289 (0.0337577); expected 171.2 (0.02)
Passed bias filter: 204 (0.023829); expected 171.2 (0.02)
Passed Vit filter: 18 (0.00210256); expected 8.6 (0.001)
Passed Fwd filter: 6 (0.000700853); expected 0.1 (1e-05)
Initial search space (Z): 8561 [actual number of targets]
Domain search space (domZ): 6 [number of targets reported over threshold]
# CPU time: 0.09u 0.00s 00:00:00.09 Elapsed: 00:00:00.04
# Mc/sec: 21336.89
//
[ok]