##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	6491_ATCACG_R1.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	135218638	
Filtered Sequences	0	
Sequence length	100	
%GC	48	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.588245793453414	31.0	30.0	33.0	28.0	33.0
2	31.788220023337317	33.0	31.0	33.0	30.0	34.0
3	32.11831721748299	33.0	31.0	33.0	31.0	34.0
4	36.163324792548195	37.0	35.0	37.0	35.0	37.0
5	36.19127765508184	37.0	35.0	37.0	35.0	37.0
6	36.23409664871791	37.0	37.0	37.0	35.0	37.0
7	36.2618322409075	37.0	37.0	37.0	35.0	37.0
8	36.282103270408626	37.0	37.0	37.0	35.0	37.0
9	38.097792273281144	39.0	39.0	39.0	35.0	39.0
10-14	38.29996434663097	39.4	38.6	39.4	35.2	39.4
15-19	39.37099892841695	41.0	39.0	41.0	36.2	41.0
20-24	39.42234856558754	41.0	39.0	41.0	36.0	41.0
25-29	39.26274434002212	41.0	39.0	41.0	36.0	41.0
30-34	39.044125252910774	40.6	39.0	41.0	35.4	41.0
35-39	38.81741841535188	40.0	38.2	41.0	35.0	41.0
40-44	38.69277644254929	40.0	38.0	41.0	35.0	41.0
45-49	38.47052305319035	40.0	38.0	41.0	34.4	41.0
50-54	38.03885316608499	40.0	37.2	41.0	33.6	41.0
55-59	37.46980116749882	39.4	35.6	41.0	33.0	41.0
60-64	36.730618811587206	38.4	35.0	40.4	31.8	41.0
65-69	35.84514289073079	36.8	34.8	39.8	31.0	41.0
70-74	34.878844799486885	35.6	34.0	38.6	29.8	40.4
75-79	33.22673050441464	34.6	32.4	36.0	28.2	38.8
80-84	33.32528733649868	35.0	33.2	35.8	29.0	37.6
85-89	32.58648179106789	35.0	33.0	35.0	28.4	36.4
90-94	31.88444527447466	34.0	32.4	35.0	26.6	35.4
95-99	30.784323329746893	34.0	31.4	35.0	22.8	35.0
100	22.97784181940954	26.0	19.0	31.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	181.0
3	436.0
4	605.0
5	1859.0
6	3887.0
7	9709.0
8	23379.0
9	42906.0
10	63314.0
11	79082.0
12	98070.0
13	110584.0
14	128753.0
15	153643.0
16	177765.0
17	201661.0
18	223273.0
19	247783.0
20	268854.0
21	295476.0
22	330313.0
23	389164.0
24	479251.0
25	581879.0
26	727031.0
27	918813.0
28	1158485.0
29	1468558.0
30	1853549.0
31	2355733.0
32	3079859.0
33	4253802.0
34	6484945.0
35	1.0717877E7
36	2.0106031E7
37	3.5751059E7
38	3.6685851E7
39	5744906.0
40	312.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.85094737652869	9.448658255929667	4.533050021868046	33.1673443456736
2	26.394721966621322	9.725436323911103	34.76176726693913	29.118074442528446
3	23.499710890446924	16.495264506361913	24.724425933058132	35.28059867013303
4	27.03971918427399	23.063917416473313	22.659737188005103	27.236626211247593
5	28.759525739343715	27.07737893351655	24.011974591845835	20.151120735293905
6	24.154479544598146	28.375085339480272	24.18333076568239	23.287104350239197
7	17.479958598773102	24.493049993301568	40.98866339864048	17.038328009284847
8	19.9459744595268	21.575199566793447	33.61441120269234	24.864414770987413
9	19.833519547800798	19.782428957759507	35.57443316357025	24.809618330869448
10-14	23.390612837758134	26.532993908751962	27.439049563399294	22.63734369009061
15-19	23.870195909545476	24.87252600805356	27.520267049878257	23.737011032522712
20-24	23.671476479328412	25.093772076620173	27.440045282186716	23.794706161864696
25-29	23.645395780205785	25.175165738451295	27.28701179877565	23.892426682567265
30-34	23.612952947411628	25.132500626559956	27.153778435221344	24.10076799080707
35-39	23.64510885806418	25.119115111578417	27.160923798596993	24.07485223176041
40-44	23.657052931499294	25.107460474843617	27.190312498840246	24.04517409481684
45-49	23.63705598253216	25.074005734836714	27.108947794194076	24.17999048843705
50-54	23.585996542659732	25.065326116096205	27.181354539473922	24.167322801770137
55-59	23.59417878473232	25.05349122064075	27.16048729909556	24.191842695531367
60-64	23.590405434422646	25.002507641308153	27.113041356569035	24.294045567700167
65-69	23.632116802623575	25.017081300828742	27.205989515808014	24.144812380739666
70-74	23.723093740777795	25.135370345231966	27.011759411323627	24.129776502666612
75-79	23.721799416611525	25.027786193318736	26.954854405915356	24.295559984154384
80-84	23.75696484434255	24.99672602096347	26.99592533067972	24.250383804014252
85-89	23.888619408198362	25.17557247019496	26.774535580474762	24.161272541131908
90-94	23.95738367635013	25.12164458866588	26.589132080051453	24.331839654932537
95-99	24.067948079509964	25.217361180681575	26.505860514989095	24.208830224819366
100	23.941719483966402	25.816836729268044	25.838240583372833	24.403203203392714
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	86.01829172220229
2	55.512796409149765
3	58.78030956057996
4	54.27634539552159
5	48.910646474637616
6	47.44158389483734
7	34.51828660805795
8	44.81038923051421
9	44.643137878670245
10-14	46.027956527848744
15-19	47.60720694206819
20-24	47.46618264119311
25-29	47.53782246277306
30-34	47.7137209382187
35-39	47.719961089824594
40-44	47.70222702631614
45-49	47.81704647096921
50-54	47.753319344429876
55-59	47.78602148026369
60-64	47.884451002122816
65-69	47.776929183363244
70-74	47.85287024344441
75-79	48.017359400765905
80-84	48.00734864835681
85-89	48.049891949330274
90-94	48.28922333128267
95-99	48.27677830432933
100	48.34492268735912
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	942.0
1	590.0
2	209.5
3	195.0
4	221.5
5	232.5
6	266.0
7	322.5
8	400.0
9	554.5
10	696.5
11	888.0
12	1210.5
13	1676.0
14	2493.5
15	3885.0
16	6163.5
17	9946.0
18	15459.0
19	24939.5
20	40526.5
21	64159.5
22	101334.0
23	148075.0
24	208578.5
25	291940.5
26	403220.5
27	539702.0
28	697069.0
29	874060.5
30	1065830.0
31	1272384.0
32	1488732.0
33	1712093.5
34	1976209.0
35	2269041.5
36	2564998.0
37	2890141.5
38	3248344.5
39	3597158.5
40	3914450.0
41	4206394.5
42	4478838.5
43	4740450.0
44	4876102.5
45	4960201.0
46	5112124.5
47	5101431.0
48	5008377.5
49	4954051.5
50	4884717.0
51	4833549.5
52	4673935.5
53	4474778.0
54	4354575.5
55	4192180.0
56	3916935.5
57	3592154.0
58	3334192.5
59	3159311.5
60	2952680.5
61	2723498.5
62	2455813.5
63	2183265.0
64	1972595.5
65	1739023.0
66	1490234.5
67	1273396.0
68	1064899.0
69	846568.0
70	640293.0
71	469434.5
72	343348.0
73	243758.5
74	170986.0
75	119338.0
76	81526.0
77	57369.5
78	38872.5
79	23951.0
80	12928.5
81	7589.0
82	4705.0
83	3073.5
84	1910.5
85	1254.5
86	937.5
87	688.0
88	463.0
89	329.0
90	260.0
91	184.5
92	107.5
93	52.5
94	30.5
95	23.0
96	19.0
97	14.5
98	10.0
99	11.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02764485765638314
2	0.005000050362879709
3	0.0
4	0.0
5	0.0
6	0.0031482346390739415
7	3.1282669775153335E-4
8	0.0
9	0.0
10-14	0.01724806605432603
15-19	0.014646353707541412
20-24	5.901553305099849E-5
25-29	0.02078293378461629
30-34	0.05734505327586571
35-39	0.0066317780837283685
40-44	0.004982892964799719
45-49	0.03332351269504726
50-54	0.011303175528213796
55-59	0.0
60-64	0.05156374966592993
65-69	0.11755731484294346
70-74	0.1785546752807849
75-79	0.1253912940611042
80-84	0.016486484651620288
85-89	0.13883470709119258
90-94	0.17673584317570185
95-99	0.08268712187442681
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.35218638E8
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	90.28842821727193
#Duplication Level	Relative count
1	100.0
2	70.61622215105906
3	50.50093782881193
4	38.06212544032206
5	29.31973100324809
6	25.48149503636946
7	22.270003202342284
8	19.223203257239582
9	17.5488357198408
10++	541.9323848300471
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	41038400	3.4397523	4.5798454	9
CCAGG	30123565	2.787739	5.692462	2
TCTTC	42092110	2.7784522	6.1312575	7
TTCTT	44047750	2.599359	5.5596905	6
CTCCA	31393635	2.5428123	7.953108	1
CCAGC	26916970	2.4647067	5.447944	2
CTTCA	34034645	2.464531	6.198888	6
TCCAG	29833015	2.4421735	6.5753307	2
CAGCT	29594335	2.4226346	5.37533	3
GGCAG	25331175	2.3692327	10.256397	1
CAGGA	25870380	2.3480082	5.1165576	3
CTCCT	31582200	2.3318698	5.775187	1
GCCAG	23779665	2.2006526	11.039944	1
CTGCT	29278065	2.1847963	5.176238	3
GGAGG	23092050	2.182838	5.0357943	1
CCCAG	23656375	2.1661434	6.4722886	1
GCTGG	25336895	2.160205	5.9483094	1
GGCTG	25184815	2.1472387	7.069738	1
CCTGG	23867955	2.0134928	5.6774864	1
GGCCA	21478430	1.9876883	7.3643875	1
TCCAC	23565855	1.9087802	5.1168017	2
GGGCA	20016200	1.8721212	6.422111	1
CTCTG	24657535	1.8400016	5.054084	1
GGGAG	19147735	1.8099909	6.5506816	1
GGGGG	18432310	1.7955368	8.678848	1
GGGGC	18157300	1.7500851	6.089531	1
GCCAC	17948590	1.6434989	7.2705626	1
GGGGA	17330995	1.6382587	6.865631	1
CCCCA	18072440	1.637379	5.2583847	1
GGCCT	18496385	1.560349	5.0157843	1
GCCTG	18434975	1.5551685	6.447894	1
GCCTC	18183420	1.5177625	5.5182734	1
TTCAA	21369990	1.5176388	5.127586	7
GTGGG	17238055	1.4853761	6.221287	1
GCCCA	16196980	1.4831092	6.492145	1
GTCCA	17911145	1.466232	9.660069	1
GGGGT	16185450	1.3946748	6.509046	1
GCCTT	17854290	1.3323281	5.0198956	1
GCCAA	14667630	1.3171952	5.203409	1
GCCAT	16058450	1.3145678	5.4617934	1
GTCAG	15828945	1.309598	7.351038	1
GTCTG	17171460	1.2950377	5.9114585	1
GTCCT	16725370	1.2480854	6.9752707	1
GCCCT	13487790	1.1258203	5.0011444	1
>>END_MODULE