Flowcell AC574DACXX Run Start 2014-08-29 Read Length 50 single end HiSeq Sequencing Kit Trueseq v3 Sequencer Software HiSeq Control Software 2.0 Gerald version 1.8 Alignment Report: Legend: Total - total reads considered ( random 10% of total reads sequenced ) Total Unique - total reads aligning to only a single location in the reference U0 - # of unique hits with 0 mismatches U1 - # of unique hits with 1 mismatches U2 - # of unique hits with 2 mismatches R012 - # of hits aligning to multiple location in the reference with 0,1 or 2 mismatches NM - # of reads without a match to the reference Mapping Algorithm : GEM allowing up to 2 mismatches Lane 1 Sample: B IN Index: AGTCAA Lab ID: 7920 Application: ChIP Seq Reference: M.musculus (mm10) Total 1802735 Total Unique 1409165 78.2% U0 1305782 72.4% U1 83509 4.6% U2 19874 1.1% R012 315222 17.5% NM 63837 3.5% ------------------------------------------------ Lane 1 Sample: 18 IN Index: AGTTCC Lab ID: 7921 Application: ChIP Seq Reference: M.musculus (mm10) Total 1801086 Total Unique 1411015 78.3% U0 1314068 73.0% U1 78382 4.4% U2 18565 1.0% R012 313695 17.4% NM 61400 3.4% ------------------------------------------------ Lane 1 Sample: D1 IN Index: ATGTCA Lab ID: 7922 Application: ChIP Seq Reference: M.musculus (mm10) Total 2131472 Total Unique 1662140 78.0% U0 1549553 72.7% U1 91308 4.3% U2 21279 1.0% R012 374566 17.6% NM 77510 3.6% ------------------------------------------------ Lane 1 Sample: D2 IN Index: CCGTCC Lab ID: 7923 Application: ChIP Seq Reference: M.musculus (mm10) Total 2028859 Total Unique 1576627 77.7% U0 1474060 72.7% U1 83338 4.1% U2 19229 0.9% R012 369443 18.2% NM 65679 3.2% ------------------------------------------------ Lane 2 Sample: B4 Index: GTCCGC Lab ID: 7924 Application: ChIP Seq Reference: M.musculus (mm10) Total 3519105 Total Unique 2823254 80.2% U0 2600965 73.9% U1 182876 5.2% U2 39413 1.1% R012 483713 13.7% NM 171496 4.9% ------------------------------------------------ Lane 2 Sample: 18 4 Index: GTGAAA Lab ID: 7925 Application: ChIP Seq Reference: M.musculus (mm10) Total 3705640 Total Unique 2999907 81.0% U0 2784315 75.1% U1 178794 4.8% U2 36798 1.0% R012 468787 12.7% NM 197199 5.3% ------------------------------------------------ Lane 2 Sample: D1 4 Index: GTGGCC Lab ID: 7926 Application: ChIP Seq Reference: M.musculus (mm10) Total 3962776 Total Unique 3274941 82.6% U0 3050778 77.0% U1 187326 4.7% U2 36837 0.9% R012 444319 11.2% NM 206032 5.2% ------------------------------------------------ Lane 2 Sample: D2 4 Index: GTTTCG Lab ID: 7927 Application: ChIP Seq Reference: M.musculus (mm10) Total 3607636 Total Unique 2939260 81.5% U0 2741293 76.0% U1 165676 4.6% U2 32291 0.9% R012 425240 11.8% NM 209998 5.8% ------------------------------------------------ Lane 2 Sample: B27 Index: CGTACG Lab ID: 7928 Application: ChIP Seq Reference: M.musculus (mm10) Total 4788393 Total Unique 3425220 71.5% U0 3094425 64.6% U1 263138 5.5% U2 67657 1.4% R012 1026777 21.4% NM 247143 5.2% ------------------------------------------------ Lane 2 Sample: 18 27 Index: GAGTGG Lab ID: 7929 Application: ChIP Seq Reference: M.musculus (mm10) Total 5043680 Total Unique 3621597 71.8% U0 3326174 65.9% U1 235886 4.7% U2 59537 1.2% R012 1078671 21.4% NM 262646 5.2% ------------------------------------------------ Lane 2 Sample: D1 27 Index: ACTGAT Lab ID: 7930 Application: ChIP Seq Reference: M.musculus (mm10) Total 3342973 Total Unique 2423366 72.5% U0 2231427 66.7% U1 155195 4.6% U2 36744 1.1% R012 692649 20.7% NM 171442 5.1% ------------------------------------------------ Lane 2 Sample: D2 27 Index: ATTCCT Lab ID: 7931 Application: ChIP Seq Reference: M.musculus (mm10) Total 5778108 Total Unique 4424864 76.6% U0 4107739 71.1% U1 261273 4.5% U2 55852 1.0% R012 1071612 18.5% NM 209788 3.6% ------------------------------------------------