Flowcell AC6GVRANXX Run Start 2015-01-13 Read Length 50 paired end HiSeq Sequencing Kit Trueseq v4 Sequencer Software HiSeq Control Software 2.2.58 Gerald version 1.8 Alignment Report: Legend: Total - total reads considered ( random 10% of total reads sequenced ) Total Unique - total reads aligning to only a single location in the reference U0 - # of unique hits with 0 mismatches U1 - # of unique hits with 1 mismatches U2 - # of unique hits with 2 mismatches R012 - # of hits aligning to multiple location in the reference with 0,1 or 2 mismatches NM - # of reads without a match to the reference Mapping Algorithm : GEM allowing up to 2 mismatches Lane 6 Read 1 Sample: B cells 1 Index: GGCTAC Lab ID: 8966 Application: Smarter-Seq Reference: M.musculus (mm10) Total 5746154 Total Unique 2951251 51.4% U0 2573381 44.8% U1 257148 4.5% U2 120722 2.1% R012 948024 16.5% NM 1756700 30.6% ------------------------------------------------ Lane 6 Read 2 Sample: B cells 1 Index: GGCTAC Lab ID: 8966 Application: Smarter-Seq Reference: M.musculus (mm10) Total 5746154 Total Unique 2822408 49.1% U0 2432953 42.3% U1 259012 4.5% U2 130443 2.3% R012 909062 15.8% NM 1809292 31.5% ------------------------------------------------ Lane 6 Read 1 Sample: B cells 2 Index: CCGTCC Lab ID: 8967 Application: Smarter-Seq Reference: M.musculus (mm10) Total 5403000 Total Unique 3138104 58.1% U0 2755722 51.0% U1 266558 4.9% U2 115824 2.1% R012 907482 16.8% NM 1269536 23.5% ------------------------------------------------ Lane 6 Read 2 Sample: B cells 2 Index: CCGTCC Lab ID: 8967 Application: Smarter-Seq Reference: M.musculus (mm10) Total 5403000 Total Unique 2978215 55.1% U0 2592834 48.0% U1 262725 4.9% U2 122656 2.3% R012 851324 15.8% NM 1402820 26.0% ------------------------------------------------ Lane 6 Read 1 Sample: Ba cells 1 Index: GAGTGG Lab ID: 8968 Application: Smarter-Seq Reference: M.musculus (mm10) Total 6415396 Total Unique 3703904 57.7% U0 3291364 51.3% U1 297925 4.6% U2 114615 1.8% R012 1190396 18.6% NM 1427274 22.2% ------------------------------------------------ Lane 6 Read 2 Sample: Ba cells 1 Index: GAGTGG Lab ID: 8968 Application: Smarter-Seq Reference: M.musculus (mm10) Total 6415396 Total Unique 3521658 54.9% U0 3073360 47.9% U1 314344 4.9% U2 133954 2.1% R012 1106227 17.2% NM 1497111 23.3% ------------------------------------------------ Lane 6 Read 1 Sample: Ba cells 2 Index: ATTCCT Lab ID: 8969 Application: Smarter-Seq Reference: M.musculus (mm10) Total 5687889 Total Unique 2844055 50.0% U0 2473530 43.5% U1 270371 4.8% U2 100154 1.8% R012 1457720 25.6% NM 1266972 22.3% ------------------------------------------------ Lane 6 Read 2 Sample: Ba cells 2 Index: ATTCCT Lab ID: 8969 Application: Smarter-Seq Reference: M.musculus (mm10) Total 5687889 Total Unique 2623711 46.1% U0 2248664 39.5% U1 263812 4.6% U2 111235 2.0% R012 1377297 24.2% NM 1431260 25.2% ------------------------------------------------ Lane 7 Read 1 Sample: Day1 1 Index: GTCCGC Lab ID: 8970 Application: Smarter-Seq Reference: M.musculus (mm10) Total 5961957 Total Unique 3311767 55.5% U0 2887318 48.4% U1 292914 4.9% U2 131535 2.2% R012 1056624 17.7% NM 1496172 25.1% ------------------------------------------------ Lane 7 Read 2 Sample: Day1 1 Index: GTCCGC Lab ID: 8970 Application: Smarter-Seq Reference: M.musculus (mm10) Total 5961957 Total Unique 3179403 53.3% U0 2733091 45.8% U1 305544 5.1% U2 140768 2.4% R012 1000512 16.8% NM 1574566 26.4% ------------------------------------------------ Lane 7 Read 1 Sample: Day1 2 Index: GTGGCC Lab ID: 8971 Application: Smarter-Seq Reference: M.musculus (mm10) Total 6438482 Total Unique 3619236 56.2% U0 3162121 49.1% U1 317856 4.9% U2 139259 2.2% R012 1070082 16.6% NM 1651091 25.6% ------------------------------------------------ Lane 7 Read 2 Sample: Day1 2 Index: GTGGCC Lab ID: 8971 Application: Smarter-Seq Reference: M.musculus (mm10) Total 6438482 Total Unique 3430370 53.3% U0 2947575 45.8% U1 332478 5.2% U2 150317 2.3% R012 989541 15.4% NM 1795998 27.9% ------------------------------------------------ Lane 7 Read 1 Sample: Day2 1 Index: CGTACG Lab ID: 8972 Application: Smarter-Seq Reference: M.musculus (mm10) Total 5925741 Total Unique 3389133 57.2% U0 2975940 50.2% U1 289585 4.9% U2 123608 2.1% R012 972624 16.4% NM 1478722 25.0% ------------------------------------------------ Lane 7 Read 2 Sample: Day2 1 Index: CGTACG Lab ID: 8972 Application: Smarter-Seq Reference: M.musculus (mm10) Total 5925741 Total Unique 3234015 54.6% U0 2791123 47.1% U1 308553 5.2% U2 134339 2.3% R012 905733 15.3% NM 1589505 26.8% ------------------------------------------------ Lane 7 Read 1 Sample: Day2 2 Index: ACTGAT Lab ID: 8973 Application: Smarter-Seq Reference: M.musculus (mm10) Total 5443403 Total Unique 3005993 55.2% U0 2625553 48.2% U1 262838 4.8% U2 117602 2.2% R012 913551 16.8% NM 1445495 26.6% ------------------------------------------------ Lane 7 Read 2 Sample: Day2 2 Index: ACTGAT Lab ID: 8973 Application: Smarter-Seq Reference: M.musculus (mm10) Total 5443403 Total Unique 2857097 52.5% U0 2462810 45.2% U1 270123 5.0% U2 124164 2.3% R012 859667 15.8% NM 1565150 28.8% ------------------------------------------------ Lane 8 Read 1 Sample: Ba cells 3 Index: GCCAAT Lab ID: 8974 Application: Smarter-Seq Reference: M.musculus (mm10) Total 5772828 Total Unique 2870378 49.7% U0 2483226 43.0% U1 287409 5.0% U2 99743 1.7% R012 1231874 21.3% NM 1538748 26.7% ------------------------------------------------ Lane 8 Read 2 Sample: Ba cells 3 Index: GCCAAT Lab ID: 8974 Application: Smarter-Seq Reference: M.musculus (mm10) Total 5772828 Total Unique 2665874 46.2% U0 2280896 39.5% U1 270722 4.7% U2 114256 2.0% R012 1164289 20.2% NM 1668590 28.9% ------------------------------------------------ Lane 8 Read 1 Sample: GMPs Index: TAGCTT Lab ID: 8975 Application: Smarter-Seq Reference: M.musculus (mm10) Total 6333308 Total Unique 3279183 51.8% U0 2780591 43.9% U1 353452 5.6% U2 145140 2.3% R012 1054075 16.6% NM 1872969 29.6% ------------------------------------------------ Lane 8 Read 2 Sample: GMPs Index: TAGCTT Lab ID: 8975 Application: Smarter-Seq Reference: M.musculus (mm10) Total 6333308 Total Unique 3124005 49.3% U0 2648433 41.8% U1 325711 5.1% U2 149861 2.4% R012 984456 15.5% NM 2001811 31.6% ------------------------------------------------ Lane 8 Read 1 Sample: GMPs GFP high Index: CTTGTA Lab ID: 8976 Application: Smarter-Seq Reference: M.musculus (mm10) Total 6900180 Total Unique 3536999 51.3% U0 3013887 43.7% U1 370900 5.4% U2 152212 2.2% R012 1153892 16.7% NM 2074658 30.1% ------------------------------------------------ Lane 8 Read 2 Sample: GMPs GFP high Index: CTTGTA Lab ID: 8976 Application: Smarter-Seq Reference: M.musculus (mm10) Total 6900180 Total Unique 3376449 48.9% U0 2868897 41.6% U1 346342 5.0% U2 161210 2.3% R012 1082838 15.7% NM 2167644 31.4% ------------------------------------------------ Lane 8 Read 1 Sample: GMPs GFP low Index: ATGTCA Lab ID: 8977 Application: Smarter-Seq Reference: M.musculus (mm10) Total 5767883 Total Unique 3172291 55.0% U0 2702717 46.9% U1 332398 5.8% U2 137176 2.4% R012 1052292 18.2% NM 1420027 24.6% ------------------------------------------------ Lane 8 Read 2 Sample: GMPs GFP low Index: ATGTCA Lab ID: 8977 Application: Smarter-Seq Reference: M.musculus (mm10) Total 5767883 Total Unique 3033521 52.6% U0 2582431 44.8% U1 308896 5.4% U2 142194 2.5% R012 983203 17.0% NM 1527472 26.5% ------------------------------------------------